Discuss the main technique used, including the principle of the technique, key steps, advantages and disadvantages and use of controls.

Neuroscience

Given below is Figure 3(A) which was presented in the Journal of Neuroinflammation (2007).

Figure 3A: Representative photomicrographs showing RT-PCR products of COX-1, COX-2, mPGES-1, and β-actin mRNAs.

Your task is specific to the figure above.

In your essay, you are required to

Discuss the main technique used, including the principle of the technique, key steps, advantages and disadvantages and use of controls.

Describe in what context the authors have used this method.

Provide a concise background including the biology that underpins what is being explored in this figure.

Explain systematically the results of this figure including the different treatment conditions and/or time points, genes analysed and the results.

Interpret and discuss key findings. Are the results expected or were there any unexpected results? Was the method appropriate for this investigation? Were appropriate controls used?

Provide key conclusions and future steps based on the figure interpretation and discussion

Discuss the main technique used, including the principle of the technique, key steps, advantages and disadvantages and use of controls.